Hi All. Are we able to manipulate the “File” pop up page via script? User wants the checkboxes to be defaulted to true upon load of the pop-up. Is this possible?

which checkbox needs to be set? Setup > COmpany Preferences allows you to set "WEB SITE HOSTING FILES ALWAYS AVAILABLE" if that helps.

The “Available Without Login” should be checked. And only for specific folders. Is that something possible?

Try that setting to see if it does what you need. I’m not sure about folder limitations.